Biotechnology Unit, Faculty of Pharmacy, October 6 University announces one of its special training programs in Clinical Diagnosis under title of
"Laboratory Diagnosis Training Course"
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This Program is including two separate modules,
**The first entitled
"Microbial and Biochemical Diagnosis"
The second entitles
"Molecular Diagnosis"
First Module: "Microbial and Biochemical Diagnosis"
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**Lab management and Quality Control
**Urine analysis
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• Sample type and preservation (early morning, midstream, random, catheter)
• Physical characteristics (odor, color, appearance, PH, specificity)
• Chemical analysis using manual protocols (protein; heat coagulation, Glucose; Benedict, ketone; Rothera`s) and urine strips
• Microscopic examination: cells; pus cells, RBCs, epithelial, crystals, casts,
others)
• Urine culture (sensitivity test); microbiology
**Hematology
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-Blood structure (plasma and formed elements; WBCs, RBCs and platelets)
-Types of WBCs
-Structure, types and function of Hemoglobin
-Difference between plasma and serum
-Types of anticoagulants (EDTA, Heparin, Na-F, Ca-Oxalate)
Blood grouping (A, B, D)
-Blood Count (Haemocytometer)
• RBCs; saline
• WBCs; gentian violet
• Platelets; Amm. Oxalate
-Differential analysis (WBSc types count); differential stain
-Erythrocytes Sedimentation Rate (ESR)
-Prothrombin time (pt) and Partial Prothrombine time (ptt)
-Anemia types detection;
• Hb; Darbkins reagent
• Haematocrit (HCT)
• Indices (MCV, MCH, MCHC)
• RBCs count
• Hemolytic (Iron, Ferretin, TIBC; total iron binding capacity)
-Hb electrophoresis
**Clinical estimation
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Liver function test
-Protein -Albumin -ALT -AST
-ALP -Billirubin (T & D); Jaundice
Kidney function test
-Urea -Creatinine -Uric Acid -Na and K
Lipid Profile
-HDL -TG -TC
Glucose and Glucose tolerance test
**Rapid tests
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Virology
-HCV -HBs-Ag
Toxicology
-Different drugs
TORCH and pregnancy tests
Autoimmune diseases
• C - reactive protein (CRP)
• Anti-Streptolysin-O (ASO)
• Rheumatoid Factor
**Duration
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5 days/ 30 credit hours
**Date
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10/7/2016 - 14/7/2016
**Fees
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700 L.E.
Module Two: "Molecular Diagnosis"
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ELISA
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Theoretical
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* Antigen- Antibody interactions
*Preparation of different types of antibody
*Types of ELISA
*ELISA data analysis
Practical
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• Tumor marker (α-Fetoprotein; AFP)
• Hormones (T3, T4 and TSH)
Molecular Biology
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Theoretical
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* Theory of Real-Time PCR (detection method, assay design,
amplification plots, Cq, melting curves, efficiency, reference genes)
* Conventional PCR VS Real-Time PCR
* Applications of Real time PCR
* Study design (sample size calculation, experimental design)
* Different chemistries of Real-Time PCR
Practical
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* mRNA synthesis and quantification
* cDNA synthesis and quantification
* Performing Real-Time PCR reaction
* Real-Time PCR data analysis and trouble shooting
**Duration
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5 days/ 30 credit hours
**Date
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24/7/2016 - 28/7/2016
**Fees
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1000 L.E
******* Hands on training, you can perform all experiments******
******* Register the two modules and pay only 1500 L.E ******
